修饰抗体 Products
赖氨酸乙酰化 (acetylation) 抗体
杭州景杰生物科技有限公司
Anti-acetyllysine rabbit pAb

Cat#:

PTM-105

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

2880.00

Price: (20 μl)

864.00


Product Description

The rabbit-derived pan anti-acetyllysine polyclonal antibody is purified with acetyl lysine agarose affinity chromatography. It specifically recognizes proteins with acetyllysine residues but not the propionyllysine or butyryllysine residues with structural similarity. Except the common applications such as Western blotting, the antibody is capable of capturing lysine acetylated peptides from protease-digesting proteins both in prokaryotic and eukaryotic cells in systematically proteomic screening.


Source/Purification

This product is produced by immunizing rabbits with acetylated BSA. Antibodies are purified by acetylated lysine agarose affinity chromatography.  


Specificity

All

Recommended Applications

ELISA, WB, IAP. Not tested in other applications 

*WB=Western blotting; IAP=Immuno affinity purification; IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:1000; IAP: 1:200; IP: 1: 25-1: 500


Important
For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

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Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity.The amino-terminal tails of core histones undergo lysine acetylationin multiple sites, termed as “histone code”.Lysine acetylation in core histone soccurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Morethan 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs)have been identified to date, while the mechanistic details of substrateselection and site specificity of these enzymes remain unclear. The regulation of histonelysine acetylation status is impaired in the pathologies of cancerand other diseases and therefore, enzymes regulating histone lysine acetylationhave become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-906.  

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.  

3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.  

4. Xu G,et al. SAHA regulates histone acetylation, Butyrylation, and protein expression in neuroblastoma. J Proteome Res. 2014 Oct 3;13(10):4211-9. doi: 10.1021/pr500497e. Epub 2014 Sep 22.

5. Zhang K,et al.Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities.J Proteomics. 2015 Jan 1;112:180-9. doi: 10.1016/j.jprot.2014.09.004. Epub 2014 Sep 16.

6. Du Y,et al.Lysine malonylation is elevated in type 2 diabetic mouse models and enriched in metabolic associated proteins.Mol Cell Proteomics. 2015 Jan;14(1):227-36. doi: 10.1074/mcp.M114.041947. Epub 2014 Nov 23.

7. Sabari BR,et al.Intracellular crotonyl-CoA stimulates transcription through p300-catalyzed histone crotonylation.Mol Cell. 2015 Apr 16;58(2):203-15. doi: 10.1016/j.molcel.2015.02.029. Epub 2015 Mar 26.

8. Wang W,et al.Suberoylanilide hydroxamic acid suppresses hepatic stellate cells activation by HMGB1 dependent reduction of NF-κB1.PeerJ.  2015 Nov 3;3:e1362. doi: 10.7717/peerj.1362. eCollection 2015.

9.  Hsu WW,et al.Sirtuins 1 and 2 Are Universal Histone Deacetylases. ACS Chem Biol. 2016 Mar 18;11(3):792-9. doi: 10.1021/acschembio.5b00886. Epub 2016 Feb 5.

10. Xie Z,et al.Metabolic Regulation of Gene Expression by Histone Lysine β-Hydroxybutyrylation.Mol Cell. 2016 Apr 21;62(2):194-206. doi: 10.1016/j.molcel.2016.03.036.

11. Goudarzi A,et al.Dynamic Competing Histone H4 K5K8 Acetylation and Butyrylation Are Hallmarks of Highly Active Gene Promoters.Mol Cell. 2016 Apr 21;62(2):169-80. doi: 10.1016/j.molcel.2016.03.014.

12. Sabari BR,et al.Intracellular crotonyl-CoA stimulates transcription through p300-catalyzed histone crotonylation.Mol Cell. 2015 Apr 16;58(2):203-15. doi: 10.1016/j.molcel.2015.02.029. Epub 2015 Mar 26.

13. Wei W,et al.Large-Scale Identification of Protein Crotonylation Reveals Its Role in Multiple Cellular Functions.J Proteome Res. 2017 Apr 7;16(4):1743-1752. doi: 10.1021/acs.jproteome.7b00012. Epub 2017 Mar 28.

14. Lu Z,et al. Bioinformatic analysis and post-translational modification crosstalk prediction of lysine acetylation.PLoS One. 2011;6(12):e28228. doi: 10.1371/journal.pone.0028228. Epub 2011 Dec 2.

15. Fang X,et al.Global analysis of lysine acetylation in strawberry leaves.Front Plant Sci. 2015 Sep 15;6:739. doi: 10.3389/fpls.2015.00739. eCollection 2015.

16. Li L,et al. SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability.Nat Commun. 2016 Jul 20;7:12235. doi: 10.1038/ncomms12235.


ELISA, WB, IAP. Not tested in other applications 

*WB=Western blotting; IAP=Immuno affinity purification; IP=Immunoprecipitation


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