修饰抗体 Products
赖氨酸乙酰化 (acetylation) 抗体
杭州景杰生物科技有限公司
Anti-acetyllysine mouse mAb (clone Kac-01)

Cat#:

PTM-101

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

2580.00

Price: (20 μl)

774.00


Product Description

The mouse monoclonal pan anti-acetyllysine antibody (clone Kac-01) is purified with Protein G affinity chromatography. It specifically recognizes proteins with acetyllysine residues but not the propionyllysine or butyryllysine residues with structural similarity. Except the common usage such as Western blotting, immunoprecipitation and immunofluorescence. The antibody has been shown in combination with other one anti-acetyllysine antibody (Cat#PTM-102, clone Kac-11) to identify most lysine acetylated peptides in both prokaryotic and eukaryotic cells in proteomics screenings.


Source/Purification

The mouse monoclonal antibody is produced by immunizing animals with acetylated BSA. Antibodies are purified by acetyllysine-conjugated agarose from media of cultured cells.  


Specificity

All

Recommended Applications

ELISA, WB, IP 

*WB=Western blotting; IP=Immunopreciptation


Recommended antibody dilution

WB: 1:1000; IP: 1:25-1: 500

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. 

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Scientific Description

The post-translational ε-amino lysine acetylation of proteins, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of protein acetylation status is impaired in the pathologies of cancer and other diseases. HDACs have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20℃. Avoid repeated freeze / thaw cycles. Storage Buffer: Antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. 


**This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1.Fritz KS,et al. Ethanol metabolism modifies hepatic protein acylation in mice. PLoS One. 2013 Sep 20;8(9):e75868. doi: 10.1371/journal.pone.0075868. eCollection 2013.

2.Yao F,et al. Detection and quantification of lysine acetyl-alteration using antibody microarray. Bioanalysis. 2013 Oct;5(20):2469-80. doi: 10.4155/bio.13.191.

3.Qi C,et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci. 2014 Mar 26;34(13):44.94-508. doi: 10.1523/JNEUROSCI.3647-13.2014.

4.Zhang K,et al. Comprehensive profiling of protein lysine acetylation in Escherichia coli. J Proteome Res. 2013 Feb 1;12(2):844-51. doi: 10.1021/pr300912q. Epub 2013 Jan 17.


ELISA, WB, IP 

*WB=Western blotting; IP=Immunopreciptation


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