修饰抗体 Products
赖氨酸苯甲酰化(benzoylation)
杭州景杰生物科技有限公司
Anti-benzoyllysine mouse mAb

Cat#:

PTM-762

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description

The pan anti-benzoyllysine(Kbz) monoclonal antibody specifically recognizes proteins with lysine benzoylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues. The antibody has been well utilized for western blotting and proteomic screening to lysine benzoylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells. 


Source/Purification

This product is produced by immunizing Balb/c mice with benzoylated BSA and the ascites was immunoaffinity purified by protein G.


Specificity

benzoyllysine

Recommended Applications

ELISA, Dot blot, Western blot.  
Recommended antibody dilution: WB: 1:1000


Recommended antibody dilution

1:1000

Important

 For  Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1  x TBS, 0.1% Tween-20 at room temperatue with gentle shaking two hours.   

 Use at an assay dependent concentration.  Optimal dilutions/concentrations should be determined by the end user.  

图片123.png

A.Dot blotting analysis on indicated amount of peptide using anti-benzoyllysine mouse mAb. The list of peptides is included in the above table.

B.Western blotting analysis on 30 μg of crude proteins from human HeLa, HepG2   and mouse N2a whole cell lysates using anti-Kbz mouse mAb (1: 1000).


Scientific Description

Lysine benzoyllysine is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine benzoylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



ELISA, Dot blot, Western blot.  
Recommended antibody dilution: WB: 1:1000

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