修饰抗体 Products
赖氨酸双甘氨酸(glycine-glycine)抗体
杭州景杰生物科技有限公司
Anti-ubiquitin mouse mAb

Cat#:

PTM-1107

Ab Type:

M

Applications:

WB

Species:

Eukaryotes

Price: (100 μl)

2580.00

Price: (20 μl)

774.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by affinity chromatography immobilized with immunogen peptides.


Source/Purification

This product is produced by immunizing mice with a synthetic peptide corresponding to amino terminus of human ubiquitin protein. Antibodies are purified by peptide affinity chromatography.


Specificity

/

Recommended Applications

ELISA, WB; Not tested in other applications

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-ubiquitin mouse mAb
 
  

Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12, COS-7 whole cell lysates using anti-ubiquitin mouse mAb (1:2000).


Scientific Description

Ubiquitin (Ub) was first identified in 1975 as a 76 amino acid protein that is highly conserved in all eukaryotic cells. Ubiquitin requires three-types of enzyme to conjugate to other proteins: Ub-activating enzyme (E1), Ub-conjugating enzymes (E2), and Ub-ligases (E3). Ubiquitin can be covalently attached to lysine (Lys) residues of target proteins either as a monomer (monoubiquitin), a polymer (polyubiquitin chains) or a linear polymer (linear polyubiquitin chains). All seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) can be ubiquitinated. The ubiquitinated peptides have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA, WB; Not tested in other applications

*WB=Western blotting



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