修饰抗体 Products
赖氨酸双甘氨酸(glycine-glycine)抗体
杭州景杰生物科技有限公司
Anti-ubiquitin rabbit pAb (NT)

Cat#:

PTM-1106

Ab Type:

P

Applications:

WB, IHC, ICC

Species:

H, R, M, MK

Price: (100 μl)

2500

Price: (20 μl)

750


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by affinity chromatography immobilized with immunogen peptides.


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to amino terminus of human ubiquitin protein. Antibodies are purified by peptide affinity chromatography.


Specificity

ubiquitin

Recommended Applications

ELISA, WB, IHC, ICC; Not tested in other applications

*WB=Western blotting    IHC=Immunohistochemistry    ICC=Immunocytochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:200    ICC: 1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 

Anti-ubiquitin rabbit pAb (NT)

A. Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12, COS-7 whole cell lysates using anti-ubiquitin rabbit pAb (1:2000).

Anti-ubiquitin rabbit pAb (NT)

B. Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling ubiquitin with PTM-1106 at 1/200 dilution. Both nuclear and cytoplasm staining on tumor cells of human neuroblastoma were observed. Counter stained with hematoxylin.

Anti-ubiquitin rabbit pAb (NT)

C. Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma cell line), labeling ubiquitin with PTM-1106 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue).

Scientific Description

Ubiquitin (Ub) was first identified in 1975 as a 76 amino acid protein that is highly conserved in all eukaryotic cells. Ubiquitin requires three-types of enzyme to conjugate to other proteins: Ub-activating enzyme (E1), Ub-conjugating enzymes (E2), and Ub-ligases (E3). Ubiquitin can be covalently attached to lysine (Lys) residues of target proteins either as a monomer (monoubiquitin), a polymer (polyubiquitin chains) or a linear polymer (linear polyubiquitin chains). All seven Lys residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) can be ubiquitinated. The ubiquitinated peptides have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


Recommended Applications

ELISA, WB, ICC, IHC; Not tested in other applications

*WB=Western blotting; IHC= Immunohistochemistry; ICC=Immunocytochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:200    ICC: 1:200


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!