修饰抗体 Products
赖氨酸丙二酰化 (malonylation) 抗体
杭州景杰生物科技有限公司
Anti-malonyllysine mouse mAb

Cat#:

PTM-902

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description
Source/Purification

This product is produced by immunizing mice with malonylated KLH conjugates and the ascites was immunoaffinity purified by protein G.


Specificity

All

Recommended Applications

ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:1000.


Recommended antibody dilution

1:1000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

图片12.png

A. Dot blotting analysis on indicated amount of peptide using anti-Kmal mAb. The list of peptides is included in the table on the right.   The result indicated that the specificity of the anti-Kmal mAb is very good.

B. Western blotting analysis on 30 μg of crude proteins from JK and 293T using anti-malonyllysine mouse mAb (1:1000).

 

Scientific Description

Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. With integrated proteomic approaches and biochemistry analysis, lysine malonylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine malonylation may provide a new paradigm in the cellular regulation, such as energy metabolism in diverse organisms.

 

Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


1. Peng,C., Lu, Z., Xie, Z., et al. Mol. Cell. Proteomics. 2011, 10, M111.012658.

2. Z., Xie., J., Dai, L., Dai, et al. Mol Cell Proteomics. 2012, 11:100-107.

3. Li, F., He, X.D., Ye, D.W., et al., 2015, Molecular Cell. 2015, 60, 661–675.

4. Zhou L,S, Wang F, Sun R,Q, et al. Scientific Reports. 2016, 17(6):811-22.


ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:1000.


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