修饰抗体 Products
赖氨酸丙二酰化 (malonylation) 抗体
杭州景杰生物科技有限公司
Anti-malonyllysine antibody

Cat#:

PTM-901

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

3680.00

Price: (20 μl)

1104.00


Product Description

The rabbit-derived pan malonyllysine antibody is purified with malonyl-lysine agarose affinity chromatography. It specifically recognizes proteins with malonyllysine residues but not other modified lysine residues with structural similarity. Except the common applications such as western blotting, the antibody is capable of capturing lysine malonylated peptides from protease-digesting proteins both in prokaryotic and eukaryotic cells in systematically proteomic screening.  


Source/Purification

This product is produced by immunizing rabbits with malonylated KLH conjugates. Antibodies are purified by malonylated-lysine agarose affinity chromatography.  


Specificity

All

Recommended Applications

ELISA, WB, IP. Not tested in other applications   

*WB=Western blotting; IP=Immunopreciptation  


Recommended antibody dilution

WB: 1:1,000; IP: 1:200

Important

For western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilution should be applied by the end user.

Anti-malonyllysine antibody

A. Dot blotting analysis on 10 ng of malonylated peptide library (lane 1), lysine malonylated BSA (lane 2), succinylated peptide library (lane 3), lysine succinylated BSA (lane 4), propionylated peptide library (lane 5) , lysine propionylated BSA (lane 6) , butyrylated peptide library (lane 7), lysine butyrylated BSA (lane 8) , non-malonylated peptide library (lane 9) and BSA (lane 10) using anti-malonyllysine rabbit pAb (1:1000). 


B. Western blotting analysis on 30 μg of crude proteins from HeLa cell with (right) or without (left) sodium malonylate treatment (20 mM, 72 hr) using anti-malonyllysine rabbit pAb (1:1000).


Scientific Description

Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. With integrated proteomic approaches and biochemistry analysis, lysine malonylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine malonylation may provide a new paradigm in the cellular regulation, such as energy metabolism in diverse organisms.


Storage & Stability

Store product at -20℃. Avoid repeated freeze / thaw cycles. Storage Buffer: Antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


**This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


1. Peng,C., Lu, Z., Xie, Z., et al. Mol. Cell. Proteomics. 2011, 10, M111.012658.  

2. Z., Xie., J., Dai, L., Dai, et al. Mol Cell Proteomics. 2012, 11:100-107.  

3. Li, F., He, X.D., Ye, D.W., et al., 2015, Molecular Cell. 2015, 60, 661


ELISA, WB, IP. Not tested in other applications   

*WB=Western blotting; IP=Immunopreciptation  


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