修饰抗体 Products
赖氨酸琥珀酰化 (succinylation) 抗体
杭州景杰生物科技有限公司
Anti-succinyllysine mouse mAb (clone 3D3)

Cat#:

PTM-419

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description

The pan Ksu mouse mAb (clone 3D3) is developed by using succinylated-KLH conjugates as immunogen. The product specifically recognizes proteins with lysine succinylated residues but not the structurally similar modified lysine residues or non-modified lysine residues. Besides common applications in Western blotting, the antibody has been well utilized for proteomic screening to lysine succinylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


Source/Purification

This product is produced by immunizing mice with succinylated KLH conjugates. Antibodies are purified by succinylated-lysine agarose affinity chromatography.  


Specificity

All

Recommended Applications

ELISA, WB, IP   

*WB=Western blotting;  IP=Immunopreciptation  


Recommended antibody dilution

WB: 1:1000; IP: 1:200.

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-succinyllysine mouse mAb (clone 3D3)

A.Dot blotting analysis on 20 ng of lysine succinylated peptide library (lane 1), lysine succinylated BSA (lane 2), selected histone lysine succinylated peptides (lane 3 to lane 8), lysine acetylated BSA (lane 9), lysine butyrylated BSA (lane 10), lysine propionylated BSA (lane 11), lysine crotonylated BSA (lane 12), unmodified lysine peptide library (13), unmodified BSA (14) using anti-succinyllysine mAb (clone 3D3, 1:1000).


B. Western blotting analysis on 30 μg of HeLa, C2C12 and COS crude proteins using anti-succinyllysine mAb (clone 3D3, 1:1000).


Scientific Description

Lysine succinylation is a recently identified novel Protein Post-translational Modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both eukaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB, IP   

*WB=Western blotting;  IP=Immunopreciptation  


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