修饰抗体 Products
赖氨酸巴豆酰化 (crotonylation)抗体
杭州景杰生物科技有限公司
Anti-crotonyllysine rabbit pAb

Cat#:

PTM-501

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

3680.00

Price: (20 μl)

1104.00


Product Description

The pan polyclonal anti-crotonyllysine antibody is produced by immunizing rabbits with crotonylated BSA. Antibodies are purified by crotonyl-lysine agarose affinity chromatography. The product specifically recognizes proteins with lysine crotonylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues. Besides common applications in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry, the antibody is with ChIP grade quality and has been well utilized for proteomic screening to lysine crotonylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.  


Source/Purification

The pan polyclonal anti-crotonyllysine antibody is produced by immunizing rabbits with crotonylated BSA. Antibodies are purified by crotonyl-lysine agarose affinity chromatography.


Specificity

All crotonylated proteins expected

Recommended Applications

ELISA, Dot blot, Western blot, IAP. 

Recommended antibody dilution: WB: 1:1000; IAP: 1:200


Recommended antibody dilution

WB: 1:1000; IAP: 1:200

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

Anti-crotonyllysine rabbit pAb

A. Dot blotting analysis on crotonylated BSA (Lane 1), acetylated BSA (Lane 2) or BSA (Lane 3) using crotonyllysine antibody. 

B. Western blotting analysis on HeLa core histone extracts, blocking with (Lane 2) or without (Lane 1) crotonylated BSA using crotonyllysine antibody (1:1000). 

C. HeLa cells were fixed, permeablized and stained using crotonyllysine antibody (1:500) for IF. Arrows indicated the chromatin structure in mitotic cells. 

D.Crotonyllysine was detected on paraffin mouse testis tubule sections during elongating steps (ES) 9 to 11 in spermatids by IHC using crotonyllysine antibody (1:200). The nuclei of ES are positive for lysine crotonylation. 


Scientific Description

Lysine crotonylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways.


Storage & Stability

Store product at -20℃. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.


**This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



1. Tan, M.J. et al (2011) Cell 146 ,1016–1028.

2. Andres, O.R. et al (2016) Biologists Ltd 10.1242/dmm.024455.


ELISA, Dot blot, Western blot, IAP. 

Recommended antibody dilution: WB: 1:1000; IAP: 1:200



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