修饰抗体 Products
赖氨酸戊二酰化(glutarylation)修饰抗体
杭州景杰生物科技有限公司
Anti-glutaryllysine mouse mAb

Cat#:

PTM-1152

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description
Source/Purification

Anti-glutaryllysine rabbit pAbpan polyclonal anti-glutaryllysine antibody is produced by immunizing rabbits with glutarylated BSA. Antibodies are purified by glutaryl-lysine agarose affinity chromatography.

 

Specificity

All

Recommended Applications

ELISA, Dot blot, Western blot.  

Recommended antibody dilution: WB: 1:1000


Recommended antibody dilution

WB: 1:1000

Important

 For  Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1  x TBS, 0.1% Tween-20 at room temperatue with gentle shaking two hours.   

 Use at an assay dependent concentration.  Optimal dilutions/concentrations should be determined by the end user.  

捕获28.PNG 

捕获29.PNG

 A.Dot blotting analysis  on indicated amount of peptide using anti-glutaryllysine mouse mAb. The list of peptides is included in the table on the right.   

 

 B.Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse N2a and rat BRL whole cell lysates using anti-glutaryllysine mouse mAb (1: 1000). 


Scientific Description

Lysine glutarylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine glutarylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. Lysine glutarylation is a SIRT5- and nutrient-regulated PTM, which impacts metabolic processes and other mitochondrial functions.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage. 



1. Tan, M.J. et al (2014) Cell Metab. 19, 605–617.

2. Li, F., He, X.D., Ye, D.W., et al., 2015, Molecular Cell. 2015, 60, 661–675.

3. Sadhukhan S., 2015, Proc Natl Acad Sci U S A. 2016 Apr 19; 113(16):4320-5.

4. Zhou L, S, Wang F, Sun R,Q, et al. EMBO Reports. 2016, 17(6):811-22.


ELISA, Dot blot, Western blot.  

Recommended antibody dilution: WB: 1:1000



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