修饰抗体 Products
赖氨酸2-羟基异丁基酰化(2-hydroxyisobutyrylation)抗体
杭州景杰生物科技有限公司
Anti-2-Hydroxyisobutyryllysine rabbit pAb

Cat#:

PTM-801

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

3680.00

Price: (20 μl)

1104.00


Product Description

The pan polyclonal anti-2-hydroxyisobutyryllysine (anti-2ohibutyryllysine) antibody is produced by immunizing rabbits with 2ohibutyrylated BSA. Antibodies are purified by 2ohibutyryl-lysine agarose affinity chromatography. The product specifically recognizes proteins with lysine 2ohibutyrylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues. Besides common applications in Western blotting, the antibody has been well utilized for proteomic screening to lysine 2ohibutyrylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.  

 

Source/Purification

The pan polyclonal anti-2-hydroxyisobutyryllysine (anti-2ohibutyryllysine) antibody is produced by immunizing rabbits with 2ohibutyrylated BSA. Antibodies are purified by 2ohibutyryllysine agarose affinity chromatography.


Specificity

All

Recommended Applications

WB, IAP   

*WB=Western blotting;  IAP=Immuno affinity preciptation  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-2-Hydroxyisobutyryllysine rabbit pAbAnti-2-Hydroxyisobutyryllysine rabbit pAb 

A. Dot blotting analysis on acetylated peptide  library (Lane 1), butyrylated peptide library (Lane 2), crotonylate peptide  library (Lane 3), unmodified peptide library (Lane 4), 2ohibutyrylated peptide  library (Lane 5), 2ohbutyrylated peptide library (Lane 6), 3ohbutyrylated  peptide library (Lane 7), 3ohibutyrylated peptide library (Lane 8) and 4ohbutyrylated  peptide library (Lane 9) using anti-2ohibutyryllysine rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse MEF, Drosophila S2 and yeast S. cerevisiae whole cell lysates using anti-2ohibutyryllysine rabbit pAb(1:2000). 


C.Mass spectrometer analysis Representative annotated MS/MS spectra of a 2ohibutyryllysine remnant-containing peptide.  Crude proteins from HeLa cell treated with 50 mM D6-2-hydroxyisobutyrate for 72 hours were digested by trypsin. The 2ohibutyryllysine remnant-containing peptides were affinity enriched by the anti-2ohibutyryllysine rabbit pAb followed by LC-ESI-MS/MS in a Q-Exactive mass spectrometry. The sequence of the representative 2ohibutyrylated peptide is indicated and the fragment ions are labeled in the MS/MS spectrum.


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, ubiquitylation, etc. 2-hydroxyisobutyrylation of lysine is a newly identified reversible modification controlling chromosome structure and gene transcription. The reversible lysine 2-hydroxyisobutyrylation has been well demonstrated in eukaryotic histones from worm to human. The unique structure and genomic localization of histone lysine 2-hydroxyisobutyrylation suggest that it is mechanistically and functionally different from histone lysine acetylation.

 

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



 1. Lunzhi D.,etal.(2014). NAT. CHEM. BIOL. 

Lysine 2-hydroxyisobutyrylation is a widely distributed active histone mark.


WB, IAP   

*WB=Western blotting;  IAP=Immuno affinity preciptation  


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