修饰抗体 Products
酪氨酸磷酸化 (phosphorylation) 抗体
杭州景杰生物科技有限公司
Anti-phosphotyrosine mouse mAb (clone 3B6)

Cat#:

PTM-701

Ab Type:

M

Applications:

WB

Species:

All

Price: (100 μl)

2580.00

Price: (20 μl)

774.00


Product Description

Anti-phosphotyrosine mouse monoclonal antibody (clone 3B6) (Anti-pTyr mAb (clone 3B6 was developed by using phosphotyramine-KLH conjugates as immunogen. This antibody can be used in western blotting to examine the modification of tyrosine phosphorylation in immunoprecipitated proteins or the overall profile of tyrosine phosphorylation in cells and tissues.  


Source/Purification

The mouse monoclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides and immunoaffinity purified by protein G.  


Specificity

All

Recommended Applications

ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


Recommended antibody dilution

WB: 1:1000; IAP: 1:200

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

 

Anti-phosphotyrosine mouse mAb (clone 3B6)   

A.Dot blotting analysis on 20 ng of tyrosine phosphorylated peptides (1-10), threonine phosphorylated peptides (11-12), serine phosphorylated peptides (13-14) and unmodified peptides (15-16) using anti-phosphotyrosine antibody (clone 3B6, 1:1000).

 

B.Western blotting analysis on 30 µg of crude proteins extracted from HeLa cells with (right) or without (left) sodium vanadate treatment (1 mM for 15 mins) using anti-phosphotyrosine antibody (clone 3B6, 1:1000).


Scientific Description

Tyrosine phosphorylation is one of the major means of cell signaling transduction in multiple pathophysiological conditions regulated by tyrosine kinase activities. In cancer, unregulated tyrosine kinase activity can drive tumor malignancy and formation by generating inappropriate proliferation and survival signals. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to these studies. The anti-phosphotyrosine antibody (clone 3B6) developed by PTM Biolabs, Inc. provides an exceptionally sensitive tool for studying tyrosine phosphorylation and its kinase activities in biomedical and preclinic studies as well as high throughput drug discovery.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


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