修饰抗体 Products
酪氨酸硝基化 (Nitrotyrosine) 抗体
杭州景杰生物科技有限公司
Anti-nitro-Tyrosine rabbit pAb

Cat#:

PTM-751

Ab Type:

P

Applications:

WB

Species:

All

Price: (100 μl)

3360.00

Price: (20 μl)

1008.00


Product Description

The rabbit-derived anti-3NT antibody is purified by nitrated tyrosine agarose affinity chromatography. It specifically recognizes proteins with tyrosine nitrated residues but not the tyrosine phosphorylated or non-modified tyrosine residues. Besides common applications in Western blotting and immunofluorescence, the antibody has been well utilized for proteomic screening to tyrosine nitrated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


Source/Purification

This product is produced by immunizing rabbits with nitrated KLH. The antiserum was immunoaffinity purified by protein A and followed with nitrotyrosine containing peptides.


Specificity

All tyrosine nitrated proteins expected

Recommended Applications

ELISA, WB, IP   

*WB=Western blotting;  IP=Immunopreciptation 


Recommended antibody dilution

WB: 1:1000

Important

For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

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Dot blotting analysis on indicated amount of peptides using 3-nitrotyrosine rabbit pAb. The list of peptides is included in the table on the right. 

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Western blot analysis on 30 μg of extracts from HeLa and MEF whole cell lysates, untreated or treated with peroxynitrite(3 mM, 30 mins) or pervanadate(3 mM, 30 mins), using anti-3-nitrotyrosine Antibody (Figure B) or anti-phospho-Tyrosine rabbit pAb (PTM-702) (Figure C).


Scientific Description

Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is very stable and so is suitable for analysis in biological sample such as plasma, lung aspirants-BALF (Broncho alveolar lining fluid) and urine. Increased nitrotyrosine levels have been associated with several physiological and pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Research shows that nitrotyrosine levels can be reduced by N-acetyl cysteine which is a precursor to glutathione, one of the body's primary endogenous antioxidants.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage. 


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA, WB, IP   

*WB=Western blotting;  IP=Immunopreciptation 


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