修饰抗体 Products
组蛋白赖氨酸泛素化(ubiquitylation)修饰抗体
杭州景杰生物科技有限公司
Anti-ubiquityl-Histone H2A (Lys119) rabbit Ab

Cat#:

PTM-1119

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

¥ 3360.00

Price: (20 μl)

1008.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by ubiquitylated histone H2A (Lys119) peptide affinity chromatography. It specifically recognizes histone H2A with ubiqitylation at Lys119.  



Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic ubiquityl peptide corresponding to residues surrounding Lys119 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by ubiquitylated histone H2A (Lys119) peptide affinity chromatography.   



Specificity

H2A

Recommended Applications
ELISA, Dot blot, WB; Not tested in other applications    
*WB=Western blotting; 
 
Recommended antibody dilution

WB: 1:2000


Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-ubiquityl-Histone H2A (Lys119) rabbit Ab

No.
Peptide No.Peptide
Lane 1H2AK119ubLane 2H2AK118ac
Lane 3H2AK118crLane 4H2AK119cr
Lane 5H2AK119un

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H2AK119ub rabbit pAb. The list of peptides is included in the table on the right.

 

Anti-ubiquityl-Histone H2A (Lys119) rabbit Ab

Figure B: Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse N2a, rat BRL whole cell lysates and mouse Liver whole tissue lysates using histone  H2AK119ub rabbit pAb (1: 2000).





















Scientific Description

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for transcriptional silencing of the developmental HOX genes and X chromosome inactivation. Histone H2A is also mono-ubiquitinated at Lys119 at sites of DNA damage. 



Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB; Not tested in other applications

*WB=Western blotting


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