修饰抗体 Products
组蛋白赖氨酸乳酸化 (lactylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-lactyl-Histone H3 (Lys18) rabbit mAb

Cat#:

PTM-1406RM

Ab Type:

Recombinant monoclonal

Applications:

WB, Dot, IHC

Species:

Human

Price: (100 μl)

4080.00

Price: (20 μl)

1500.00


Product Description

The recombinant rabbit monoclonal antibody is purified with protein A-conjugated agarose. It specifically recognizes histone H3 with lactylation at Lys18.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic lactyl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein A-conjugated agarose.  


Specificity

H3

Recommended Applications

Dot blot,WB; Not tested in other applications

*WB=Western blotting

 
 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  




Anti-lactyl-Histone H3 (Lys18) rabbit mAb



No.
Peptide No.Peptide
Lane 1H3K18laLane 2H3K14cr
Lane 3H3K14bzLane 4H3K14la
Lane 5H3K18buLane 6H3K18cr
Lane 7H3K18hibLane 8H3K18bz
Lane 9H3K23crLane 10H3K23bz
Lane 11H3K23laLane 12H3K27cr





Lane 13H3K27laLane 14H3K18un

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H3K18la rabbit mAb. The list of peptides is included in the table on the right.

 

Anti-lactyl-Histone H3 (Lys18) rabbit mAb

Figure B: Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium lactate (100mM, 24 hours) whole cell lysates using lactyl-histone H3 (Lys18) rabbit mAb (1: 2000).


Anti-lactyl-Histone H3 (Lys18) rabbit mAb

Figure CImmunohistochemical analysis of paraffin-embedded human breast cancer tissue, labeling H3K18la with PTM-1406RM at 1/1000 dilution. The PTM-1406RM was pretreated with un-lactyl-Histone H3 peptide (Panel A) and lactyl-Histone H3 (Lys18) (Panel B) site-specific peptide for 2 hours. Nuclear

staining on tumor cells of human breast cancer in Panel A, no staining in Panel B. 

Counter stained with hematoxylin.Heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0)






Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Lactyl-Histone H3 (Lys18) rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Zhang D, Tang ZY, Huang H, et al.  Nature2019, 574:575-580


Dot, WB; Not tested in other applications

*WB=Western blotting


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