修饰抗体 Products
组蛋白赖氨酸乳酸化 (lactylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-lactyl-Histone H3 (Lys56) rabbit mAb

Cat#:

PTM-1421RM

Ab Type:

Recomninant monoclonal

Applications:

WB, Dot, ELISA

Species:

Human

Price: (100 μl)

4080.00

Price: (20 μl)

1500.00


Product Description

The recombinant rabbit monoclonal antibody is purified with protein A-conjugated agarose. It specifically recognizes histone H3 with lactylation at Lys56.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic lactyl peptide corresponding to residues surrounding Lys56 of human histone H3. Antibodies are purified by protein A-conjugated agarose.  


Specificity

H3

Recommended Applications

ELISA, Dot blot,WB; Not tested in other applications

*WB=Western blotting; 

 
 

Recommended antibody dilution

WB: 1:1000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  




Anti-lactyl-Histone H3 (Lys56) rabbit mAb


No.
Peptide No.Peptide
Lane 1H3K56laLane 2H3K56cr
Lane 3H3K56hibLane 4H3K56un

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H3K56la rabbit mAb. The list of peptides is included in the table above.

 

Anti-lactyl-Histone H3 (Lys56) rabbit mAb

Figure C: Western blotting analysis on 30 μg of human HeLa whole cell lysates with (right) or without (left) treatment of sodium lactate (100mM, 24 hours) using lactyl-histone H3 (Lys56) rabbit mAb (1: 1000). 


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Lactyl-Histone H3 (Lys14) rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Zhang D, Tang ZY, Huang H, et al.  Nature2019, 574:575-580


ELISA, WB; Not tested in other applications

*WB=Western blotting


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