修饰抗体 Products
组蛋白赖氨酸乳酸化 (lactylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-lactyl-Histone H3 (Lys18) rabbit pAb

Cat#:

PTM-1406

Ab Type:

P

Applications:

WB, Dot, IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

4080.00

Price: (20 μl)



Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by lactylated histone H3(Lys18) peptide affinity chromatography. It specifically recognizes histone H3 with lactylation at Lys18.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic lactyl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by lactylated histone H3 (Lys18) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

Dot blot,WB, IHC; Not tested in other applications

*WB=Western blotting; IHC=immunohistochemitry

 
 

Recommended antibody dilution

WB: 1:2000,IHC: 1:100

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  




Anti-lactyl-Histone H3 (Lys18) rabbit pAb

No.
Peptide No.Peptide
Lane 1H3K18laLane 2H3K9cr
Lane 3H3K9laLane 4H3K14cr
Lane 5H3K14laLane 6H3K18ac
Lane 7H3K18prLane 8H3K18bu
Lane 9H3K18crLane 10H3K23la
Lane 11H3K23crLane 12H3K27la





Lane 13H3K27crLane 14H3K18un

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H3K18la rabbit pAb. The list of peptides is included in the table on the right.

 

Anti-lactyl-Histone H3 (Lys18) rabbit pAb

Anti-lactyl-Histone H3 (Lys18) rabbit pAb

Figure B: Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium lactate (100mM, 24 hours) , mouse N2a and rat BRL whole cell lysates using lactyl-histone H3 (Lys18) rabbit pAb (1: 2000).


                       Anti-lactyl-Histone H3 (Lys18) rabbit pAb

Figure C

Immunohistochemical analysis of paraffin-embedded mouse colon tissue, labeling H3K18lac with PTM-1406 at 1/100 dilution. Nuclear staining on goblet cells of mouse colon is observed. Counter stained with hematoxylin.







Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Lactyl-Histone H3 (Lys18) rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Zhang D, Tang ZY, Huang H, et al.  Nature2019, 574:575-580


Dot, WB,IHC; Not tested in other applications

*WB=Western blotting IHC=Immunohistochemistry


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