修饰抗体 Products
组蛋白赖氨酸乳酸化 (lactylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-lactryl-Histone H4 (Lys5) rabbit pAb

Cat#:

PTM-1407

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

4080.00

Price: (20 μl)

1500.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by lactylated histone H4(Lys5) peptide affinity chromatography. It specifically recognizes histone H4 with lactylation at Lys5.   


Source/Purification

This product is produced by immunizing rabbits with a synthetic lactyl peptide corresponding to residues surrounding Lys5 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by lactylated histone H4 (Lys5) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, Dot blot,WB; Not tested in other applications

*WB=Western blotting; 

 
 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  


    NO.Peptide NO.Peptide
Anti-lactryl-Histone H4 (Lys5) rabbit pAbLane 1H4K5la Lane 2H4K5ac
Lane 3H4K5pr Lane 4H4K5cr
Lane 5H4K5hib Lane 6H4K5bhb
Lane 7H4K8ac Lane 8H4K8pr
Lane 9H4K8cr Lane 10H4K8hib
 Lane 11H4K8bhb Lane 12H4K8la
 Lane 13H4K12ac Lane 14H4K12cr
 Lane 15H4K12hib Lane 16H4K12bhb
 Lane 17H4K12la Lane 18H4K5un
 

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H4K5la rabbit pAb. 

The list of peptides is included in the table on the right.


Anti-lactryl-Histone H4 (Lys5) rabbit pAb

Figure B: Western blotting on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium lactate (100mM, 24 hours) using lactyl-histone H4 (Lys5) rabbit pAb (1: 2000).

Anti-lactryl-Histone H4 (Lys5) rabbit pAb

Figure C: Western blotting analysis on 30 μg of crude proteins from human HeLa, Rabbit N2a, rat BRL whole cell lysates using lactyl-histone H4 (Lys5) rabbit pAb (1: 2000).


 


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Lactyl-Histone H4 (Lys12) rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Zhang D, Tang ZY, Huang H, et al.  Nature2019, 574:575-580


ELISA, WB; Not tested in other applications

*WB=Western blotting


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