修饰抗体 Products
组蛋白赖氨酸乳酸化 (lactylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-lactryl-Histone H4 (Lys5) mouse mAb

Cat#:

PTM-1409

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

4080.00

Price: (20 μl)

1500.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by lactylated histone H4(Lys5) peptide affinity chromatography. It specifically recognizes histone H4 with lactylation at Lys5.  


Source/Purification

This product is produced by immunizing mice with a synthetic lactyl peptide corresponding to residues surrounding Lys5 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by lactylated histone H4 (Lys5) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, Dot blot,WB; Not tested in other applications

*WB=Western blotting; 

 
 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  




        Lactryl-Histone H4 (Lys5) mouse mAb

No.
Peptide No.Peptide
Lane 1H4K5lacLane 2H4K5ac
Lane 3H4K5prLane 4H4K5bu
Lane 5H4K5crLane 6H4K5hib
Lane 7H4K5bhbLane 8H4K8bu
Lane 9H4K8lacLane 10H4K12lac
Lane 11H4K16lacLane 12H4K5un

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H4K12la rabbit pAb. The list of peptides is included in the above table.


 

anti-actryl-Histone H4 (Lys5) mouse mAb

Figure B: Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium lactate (100mM, 24 hours) using lactryl-histone H4 (Lys5) mouse mAb (1: 2000). 

anti-actryl-Histone H4 (Lys5) mouse mAb

Figure C: Western blotting analysis on 30 μg of crude proteins from mouse N2a, rat BRL whole cell lysates and mouse spleen whole tissue lysates using lactryl-histone H4 (Lys5) mouse mAb (1: 2000).


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Lactyl-Histone H4 (Lys12) rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Zhang D, Tang ZY, Huang H, et al.  Nature2019, 574:575-580


ELISA, WB; Not tested in other applications

*WB=Western blotting


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