修饰抗体 Products
组蛋白赖氨酸乳酸化 (lactylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-lactyl-Histone H4 (Lys8) rabbit pAb

Cat#:

PTM-1415

Ab Type:

P

Applications:

WB, Dot, ELISA, IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

¥ 4080.00

Price: (20 μl)

¥ 1500.00

国外价格

Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by lactylated histone H4(Lys8) peptide affinity chromatography. It specifically recognizes histone H4 with lactylation at Lys8.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic lactyl peptide corresponding to residues surrounding Lys8 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by lactylated histone H4 (Lys8) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, Dot blot, WB, IHC; Not tested in other applications

*WB=Western blotting;  IHC=Immunohistochemistry

 
Recommended antibody dilution

WB: 1:2000    IHC: 1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Lactyl-Histone H4 (Lys8) rabbit pAb

No.
Peptide No.Peptide
Lane 1H4K8laLane 2H4K5hib
Lane 3H4K5laLane 4H4K8ac

Figure A: Dot blotting analysis on indicated amount of peptides using anti-H4K8la rabbit 

pAb. The list of peptides is included in the 

table on the right.

Lane 5H4K8prLane 6H4K8bu
Lane 7H4K8crLane 8H4K8bhb
Lane 9H4K8hibLane 10H4K12hib
Lane 11H4K12laLane 12H4K8un


Lactyl-Histone H4 (Lys8) rabbit pAb

Figure B: Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium lactate (100mM, 24 hours) using lactyl-histone H4 (Lys8) rabbit pAb (1: 2000)

Lactyl-Histone H4 (Lys8) rabbit pAb

Figure C: Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse N2a, rat BRL whole cell lysates    using lactyl-histone H4 (Lys8) rabbit pAb (1: 2000).

Anti-lactyl-Histone H4 (Lys8) rabbit pAb

Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling H4K8ac with PTM-1415 at 1/200 dilution.

Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin.





















Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Lactyl-Histone H4 (Lys8) rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Zhang D, Tang ZY, Huang H, et al.  Nature2019, 574:575-580


Recommended Applications

ELISA, Dot blot, WB, IHC; Not tested in other applications

*WB=Western blotting;  IHC=Immunohistochemistry

 
 

Recommended antibody dilution

WB: 1:2000    IHC: 1:200


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