修饰抗体 Products
赖氨酸乳酸化(lactylation)抗体
杭州景杰生物科技有限公司
Anti-L-lactyllysine rabbit mAb

Cat#:

PTM-1401 RM

Ab Type:

rabbit monoclonal

Applications:

Price (100 μl):

WB, IHC, ICC

¥4200.00

Species:


All species is expected



Product Description

The pan rabbit monoclonal anti-L-lactyllysine antibody was produced by immunizing rabbits with L-lactylated BSA. Antibodies were purified by protein A. The product specifically recognizes proteins with lysine lactylated residues but not the structurally-similar modified or non-modified lysine residues. The applications of this antibody include Western blotting, immunocytochemistry and immunohistochemistry. In addition, it is well utilized for proteomic screening to lysine lactylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


Source/purification

The pan rabbit monoclonal anti-L-lactyllysine antibody was produced by immunizing rabbits with L-lactylated BSA. Antibodies are purified by protein A.



Specificity

Anti-L-lactyllysine antibody detects proteins which are post-translationally modified with lactylation at lysine residues, but does not cross-react with the acetylated, propionylated, butyrylated, succinylated, crotonylated and unmodified peptide library.



Recommended Applications

ELISA, Dot blot, Western blot, Immunohistochemistry, Immunocytochemistry

Recommended antibody dilution

WB: 1:2000, IHC:1:100, ICC/IF: 1:100

Important NOTES

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.


 

 

 

 

NO.

PeptideNO.Peptide

Anti-L-lactyllysine rabbit mAb

Lane 1

Klac library

Lane 2

Kac library

Lane 3

Kpr library

Lane 4

Kbu library

Lane 5

Kcr library

Lane 6

Kmal library

Lane 7

Khib library

Lane 8

Ksu library

  Lane 9control library

 

 

 

Figure A:Dot blotting analysis on indicated amount of peptides using anti-Klac rabbit mAb. The list of peptides is included in the above table.

Anti-L-lactyllysine rabbit mAb

 

Figure B: Western blotting analysis on 30 μg   of crude proteins from HeLa whole cell with (+) or without (-) treatment of sodium lactate (100 mM, 24hr) using anti-Kla rabbit mAb (1: 1000).



Anti-L-lactyllysine rabbit mAb

 

Anti-L-lactyllysine rabbit mAb

Figure C: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue, labeling anti-Kla with PTM-1401 RM at 1/100 dilution. 

Nuclear staining on tumor cells of human colon cancer was observed. Counter stained with hematoxylin.Heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

 

Figure D:Immunocytochemical analysis of Raji (Burkitt's lymphoma cell line), labeling anti-Kla with PTM1401RM at 1/100 dilution. Both nuclear and cytoplasmic stainingon Raji cell line. Nuclei were stained with DAPI (blue). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. 


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Anti-L-lactyllysine rabbit pAb

Storage & Stability:

The antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.

**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.



Zhang D, Tang ZY. et al (2019). Metabolic regulation of gene expression by histone lactylation. Nature, vol. 574, pages 575–580 .

ELISA, WB, IHC, ICC. Not tested in other applications

*WB=Western blotting; ICC: Immunocytochemistry; IHC=Immunohistochemistry


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