修饰抗体 Products
赖氨酸乳酸化(lactylation)抗体
杭州景杰生物科技有限公司
Anti-L-lactyllysine rabbit pAb

Cat#:

PTM-1401

Ab Type:

P

Applications:

Price (100 μl):

WB, IAP, IHC

¥4200.00

Species:


All



Product Description

The pan polyclonal anti-L-lactyllysine antibody was produced by immunizing rabbits with L-lactylated BSA. Antibodies were purified by l-lactyllysine agarose affinity chromatography. The product specifically recognizes proteins with lysine lactylated residues but not the structurally-similar modified or non-modified lysine residues. The applications of this antibody include Western blotting, immunoprecipitation and immunohistochemistry. In addition, it is well utilized for proteomic screening to lysine lactylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


Source/purification

The pan polyclonal anti-L-lactyllysine antibody is produced by immunizing rabbits with lactylated BSA. Antibodies are purified by lactyl-lysine agarose affinity chromatography.


Specificity

Anti-L-lactyllysine antibody detects proteins which are post-translationally modified with lactylation at lysine residues, but does not cross-react with the acetylated, propionylated, butyrylated, succinylated, crotonylated and unmodified peptide library.

Recommended Applications

ELISA, Dot blot, Western blot, Immunohistochemistry

Recommended antibody dilution

WB: 1:2000, IHC:1:200

Important NOTES

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.


 

 

 

 

NO.

PeptideNO.Peptide

Anti-L-lactyllysine rabbit pAb

Lane 1

Klac library

Lane 2

Kac library

Lane 3

Kpr library

Lane 4

Kbu library

Lane 5

Ksu library

Lane 6

Kcr library

Lane 7

Kme3 library

Lane 8

control library

 

 

 

Figure A:Dot blotting analysis on indicated amount of peptides using anti-Klac rabbit pAb. The list of peptides is included in the above table.

Anti-L-lactyllysine rabbit pAb

Anti-L-lactyllysine rabbit pAb

 

Anti-L-lactyllysine rabbit pAb

Figure B:  Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell with (+) or without (-) treatment of sodium lactate (100 mM, 24hr), 30μg whole  tissue lysates of mouse kidney and mouse spleen using anti-Klac rabbit pAb (1: 1000).

 

Figure C: Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse N2a and Rat BRL whole cell lysates using anti-Klac rabbit pAb (1: 1000)


Anti-L-lactyllysine rabbit pAb

 

Anti-L-lactyllysine rabbit pAb

Figure D: Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue labeling L-lactyllysine with PTM-1401 at 1/200 dilution. Cytoplasmic and nuclear staining on tumor cells of human neuroblastoma. Counter stained with hematoxylin.

 

Figure E:Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling L-lactyllysine with PTM-1401 at 1/200 dilution. Cytoplasmic staining on tumor cells of human hepatocellular carcinoma. Counter stained with hematoxylin.


Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

Anti-L-lactyllysine rabbit pAb

Storage & Stability:

The antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.

**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.



Zhang D, Tang ZY. et al (2019). Metabolic regulation of gene expression by histone lactylation. Nature, vol. 574, pages 575–580 .

ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno-affinity precipitation; IHC=Immunohistochemistry


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!