修饰抗体 Products
β-actin抗体
杭州景杰生物科技有限公司
β-actin mouse mAb

Cat#:

PTM-1012

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Scientific Description:

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α-and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells . Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. It has been reported that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle. Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome dependent muscle proteolysis.

 

β-actin mouse mAb

Figure A: Western blotting analysis on 30 μg of crude proteins from HeLa, N2a, BRL, COS-7 whole cell lysates and mouse kidney whole tissue lysates using β-actin mouse mAb (1:2000).


Storage & Stability:

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


β-actin mouse mAb


ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000 


NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


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