修饰抗体 Products
β-Tubulin抗体
杭州景杰生物科技有限公司
β-Tubulin mouse mAb

Cat#:

PTM-1011

Ab Type:

M

Applications:

WB

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

This product is produced by immunizing mice with a synthetic peptide corresponding to N terminus of β-Tubulin protein (uniprot ID: P07407). The antibody was purified from antisera by immunoaffinity chromatography using antigens.


Source/Purification

This product is produced by immunizing mice with a synthetic peptide corresponding to residues surrounding N terminus of human β-Tubulin protein  Antibodies are purified by protein A-conjugated agarose followed by peptide affinity chromatography.  


Specificity

β-Tubulin

Recommended Applications

WB; Not tested in other applications

*WB=Western blotting; 

 
 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  


β-Tubulin mouse mAb

 

Western blotting analysis on 30 μg of crude proteins from HeLa, BRL, COS-7, N2a whole cell lysates and mouse liver, kidney whole tissue lysates using β-Tubulin mouse mAb (1:2000).

 


Scientific Description

Alpha and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division.

In eukaryotes there are six members of the tubulin superfamily, although not all are present in all species. Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin (with a mass of ~42 kDa). In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature.

β-Tubulin rabbit pAb


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Recommended Applications:  

ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000


NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


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