修饰抗体 Products
赖氨酸乙酰化 (acetylation) 修饰ChIP级抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H3 (Lys4) mouse pAb ChIP Grade

Cat#:

GC-178

Ab Type:

M

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with lysine acetylation at Lys4.


Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H3 (Lys4) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ChIP, IP. Not tested in other applications 

*IP=Immunoprecipitation        ChIP=Chromatin precipitation


Recommended antibody dilution

IP: 1:50 ChIP: 1:25 (4 μl of antibody for 1x 105 HeLa)

Important

 Use  at an assay dependent concentration. Optimal dilutions/concentrations should be  determined by the end user.   

Anti-acetyl-Histone H3 (Lys4) mouse pAb ChIP Grade 

A. Dot  blotting analysis on indicated amount of acetylated H3 peptide at Lys4 (lane 1),  mono-methylated H3 peptide at Lys4 (lane 2), di-methylated peptide at Lys4  (lane 3), tri-methylated peptide at Lys4 (lane 4), crotonylated H3 peptide at  Lys4 (lane 5), 2ohibutylated H3 peptide at Lys4 (lane 6), acetylated peptide at  Lys9 (lane 7), butyrylated peptide at Lys9 (lane 8), crotonylated peptide at  Lys9 (lane 9), mono-methylated H3 peptide at Lys9 (lane 10), di-methylated  peptide at Lys9 (lane 11) and unmodified H3 peptide at Lys4 (lane 12) using acetyl-histone  H3 (Lys4) mouse mAb. 


B. Sonicated chromatin  prepared from 1x 10HeLa  cells treated with sodium  butyrate was subjected to chromatin immunoprecipitation (ChIP) using  either 4µl acetyl-histone H3 (Lys4)  mouse mAb or 1 μg of normal mouse IgG as a negative control. Real time quantitative  PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH Coding D2, GAPDH promoter andα Satellite. Data are presented as  enrichment of each IPed sample relative to total amount of input chromatin at  each amplicon.


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiati on and nuclear import et al. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ChIP, IP. Not tested in other applications 

*IP=Immunoprecipitation        ChIP=Chromatin precipitation


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