修饰抗体 Products
赖氨酸乙酰化 (acetylation) 修饰ChIP级抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H3 (Lys18) mouse mAb ChIP Grade

Cat#:

GC-168

Ab Type:

M

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H3 (Lys18) peptide affinity chromatography. It specifically recognizes histone H3 with acetylation at Lys18. 


Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H3 (Lys18) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ChIP, IP. Not tested in other applications   

*IP=Immunoprecipitation        ChIP=Chromatin precipitation  


Recommended antibody dilution

IP: 1:25 ChIP: 1:50 (2 μl of antibody for 1x 105 HeLa)

Important

 Use  at an assay dependent concentration. Optimal dilutions/concentrations should be  determined by the end user.  

 Anti-acetyl-Histone H3 (Lys18) mouse mAb ChIP Grade

A.Dot blotting analysis on indicated amount of acetylated H3 peptide at Lys18 (lane 1);acetylated peptide at Lys14 (lane 2), propionylated H3 peptide at Lys18 (lane 3),  butyrylated peptide at Lys18 (lane 4), crotonylated peptide at Lys18 (lane 5), and  unmodified H3 peptide at Lys18 (lane 6) using acetyl-histone H3 (Lys18) mouse mAb.  


B. Sonicated chromatin prepared from 1x 10 HeLa cells treated with sodium butyrate  was subjected to chromatin immunoprecipitation (ChIP) using serial dilutions of Acetyl-Histone H3 (Lys18) mouse mAb or  1 μg of normal mouse IgG as a negative control. Real time quantitative PCR was  performed on immunoprecipitated DNA using primers specific for the human β-globin, GAPDH Promoter and MyoD1 Exon 1 .Data are presented as enrichment of each  IPed sample relative to total amount of input chromatin at each amplicon.  


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli. It plays vital roles in the regulation of many cellular processes including chromatin dynamics, transcriptional activities, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H2B is primarily acetylated at Lys5, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The level of  histone lysine acetylation has been found to be impaired in cancers and other diseases, therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw cycles. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.  

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ChIP, IP. Not tested in other applications   

*IP=Immunoprecipitation        ChIP=Chromatin precipitation  


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