修饰抗体 Products
赖氨酸乙酰化 (acetylation) 修饰ChIP级抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H4 (Lys16) rabbit pAb ChIP Grade

Cat#:

GC-132

Ab Type:

P

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H4 (Lys16) peptide affinity chromatography. It specifically recognizes histone H4 with lysine acetylation at Lys16.


Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys16 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H4 (Lys16) peptide affinity chromatography.


Specificity

H4

Recommended Applications

ChIP, IP. Not tested in other applications 

*IP=Immunoprecipitation        ChIP=Chromatin precipitation


Recommended antibody dilution

IP: 1:25 ChIP: 1:25 (4 μl of antibody for 1x 105 HeLa)

Important

 Use  at an assay dependent concentration. Optimal dilutions/concentrations should be  determined by the end user.  

 Anti-acetyl-Histone H4 (Lys16) rabbit pAb ChIP Grade 

A. Dot  blotting analysis on indicated amount of acetylated H4 peptide at Lys16 (lane 1),  propionylated H4 peptide at Lys16 (lane2), butyrylated H4 peptide at Lys16  (lane 3), crotonylated peptide at Lys16 (lane 4), acetylated H4 peptide at Lys12  (lane 5), propionylated H4 peptide at Lys12 (lane 6), butyrylated H4 peptide at  Lys12 (lane 7), acetylated H4 peptide at Lys8 (lane 8), butyrylated H4 peptide  at Lys8 (lane 9), crotonylated peptide at Lys8 (lane 10) and unmodified H4  peptide at Lys16 (lane 11) using acetyl-histone H4 (Lys16) rabbit pAb.   

 

B. Sonicated chromatin prepared from 1x 105 HeLa cells treated with sodium butyrate was subjected to  chromatin immunoprecipitation (ChIP) using either 4µl acetyl-histone H4 (Lys16) rabbit pAb or 1 μg of normal rabbit  IgG as a negative control. Real time quantitative PCR was performed on  immunoprecipitated DNA using primers specific for the human GAPDH Coding D2 and α Satellite. Data are presented as enrichment  of each IPed sample relative to total amount of input chromatin at each  amplicon.


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation and nuclear import et al. In most species, histone H2A is primarily acetylated at Lys16, 9, 15 and 36; H2B is primarily acetylated at Lys16, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ChIP, IP. Not tested in other applications 

*IP=Immunoprecipitation        ChIP=Chromatin precipitation


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