修饰抗体 Products
组蛋白赖氨酸甲基化(methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-mono-methyl-Histone H3 (Lys18) rabbit pAb ChIP Grade

Cat#:

GC-628

Ab Type:

P

Applications:

ChIP, IP

Species:

Eukaryotes

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by mono-methylated histone H3 at Lys18 peptide affinity chromatography. It specifically recognizes histone H3 with mono-methylation at Lys18.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Lys18 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by mono-methylated histone H3 (Lys18) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ChIP, IP. Not tested in other applications   

*IP=Immunoprecipitation        ChIP=Chromatin precipitation  


Recommended antibody dilution

IP: 1:25 ChIP: 1:25 (4 μl of antibody for 1x 105 HeLa)

Important

 Use  at an assay dependent concentration. Optimal dilutions/concentrations should be  determined by the end user.  

 Anti-mono-methyl-Histone H3 (Lys18) rabbit pAb ChIP Grade

A.Dot blotting analysis on indicated amount of mono-methylated H3 peptide at Lys18 (lane  1), mono-methylated peptide at Lys14 (lane2), di-methylated peptide at Lys14 (lane3);2ohibutyrylated peptide at Lys14 (lane4), 3ohbutyrylated peptide at Lys14 (lane5);tri-methylated peptide at Lys18 (lane6), 2ohibutyrylated peptide at Lys18  (lane7), 3ohbutyrylated peptide at Lys18 (lane8), mono-methylated peptide at  Lys23 (lane9), di-methylated peptide at Lys23 (lane10), tri-methylated peptide  at Lys23 (lane11), 2ohibutyrylated peptide at Lys23 (lane12), 3ohbutyrylated  peptide at Lys23 (lane13), and unmodified H3 peptide at Lys18 (lane 14) using mono-methyl-histone H3 (Lys18)  rabbit pAb.    


B. Sonicated chromatin prepared from 1 x 105 HeLa  cells was subjected to chromatin immunoprecipitation (ChIP) using either 4µl Mono-methyl-Histone H3 (Lys18) rabbit  pAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was  performed on immunoprecipitated DNA using primers specific for the human β-globin, MyoD1 Exon 1, RPL30 Exon 3, and GAPDH  Promoter. Data are presented as enrichment of each IPed sample relative to  total amount of input chromatin at each amplicon.    


Scientific Description

The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79), and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs). The level of methyllysineis found impaired in the pathologies of cancers and other diseases, therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.  

 

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ChIP, IP. Not tested in other applications   

*IP=Immunoprecipitation        ChIP=Chromatin precipitation  

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