修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H2A (Lys15) mouse mAb

Cat#:

PTM-175

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat,Pharbitis

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated Histone H2A at Lys15 peptide affinity chromatography. It specifically recognizes Histone H2A with acetylation at Lys15.  


Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys15 of human Histone H2A. Antibodies are purified by protein G-conjugated agarose followed by acetylated Histone H2A (Lys15) peptide affinity chromatography.  


Specificity

H2A

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;   

Recommended antibody dilution: WB: 1:2000 while cell is treated by TSA and 1:1000 on tissue or untreated cell lysate.  


Recommended antibody dilution

WB: 1:2000 while cell is treated by TSA and 1:1000 on tissue or untreated cell lysate.

Important

 For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.Anti-acetyl-Histone H2A (Lys15) mouse mAb

A.Dot blotting analysis on indicated amount of acetylated H2A peptide at Lys15 (lane 1), acetylated H2A peptide at Lys9 (lane 2), succinylated H2A peptide at Lys9 (lane 3), 2ohibutyrylated H2A peptide at Lys9 (lane 4), 3ohbutyrylated H2A peptide at Lys9 (lane 5), acetylated H2A peptide at Lys13 (lane 6), acetylated H2A peptide at Lys36 (lane 7), crotonylated H2A peptide at Lys36 (lane 8), 2ohibutyrylated H2A peptide at Lys36 (lane 9), 3ohbutyrylated H2A peptide at Lys36 (lane 10), and unmodified H2A peptide at Lys15 (lane 11) using acetyl-Histone H2A (Lys15) mouse mAb.   


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (Fig B. right) or without (Fig B. left) treatment of Trichostatin A (500ng, 4 hours) using acetyl-Histone H2A (Lys15) mouse mAb (1:2000).  


C. Western blotting analysis on 30 μg of crude proteins from MCF-7 whole cell lysates and mouse liver tissue lysate using acetyl-Histone H2A (Lys15) mouse mAb (1:1000).  


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.   


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA, WB; Not tested in other applications  

*WB=Western blotting;   

Recommended antibody dilution: WB: 1:2000 while cell is treated by TSA and 1:1000 on tissue or untreated cell lysate.  


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