修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H2B (Lys20) mouse mAb

Cat#:

PTM-155

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H2B (Lys20) peptide affinity chromatography. It specifically recognizes histone H2B with acetylation at Lys20.  

Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys20 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H2B (Lys20) peptide affinity chromatography.  

Specificity

H2B

Recommended Applications

ELISA, WB; Not tested in other applications   

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

 Anti-acetyl-Histone H2B (Lys20) mouse mAb

A. Dot  blotting analysis on indicated amount of acetylated H2B peptide at Lys20 (lane 1),  acetylated H2B peptide at Lys16 (lane 2), butyrylated H2B peptide at Lys20  (lane 3) , crotonylated H2B peptide at Lys20(lane 4) and unmodified H2B peptide  at Lys20 (lane 5) using acetyl-histone H2B (Lys20) mouse mAb.  


B. Western blotting analysis on 30 μg of crude proteins  from HeLa whole cell lysates with (lane 2) or without (lane1) treatment of sodium  butyrate (30 mM, 4 hours) using acetyl-histone H2B (Lys20) mouse mAb (1:2000).  

 

Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli. It plays vital roles in the regulation of many cellular processes including chromatin dynamics, transcriptional activities, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H2B is primarily acetylated at Lys5, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The level of  histone lysine acetylation has been found to be impaired in cancers and other diseases, therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC.



ELISA, WB; Not tested in other applications   

*WB=Western blotting  


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