修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H2B (Lys23) mouse mAb

Cat#:

PTM-174

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat, Rice

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H2B (Lys23) peptide affinity chromatography. It specifically recognizes histone H2B with acetylation at Lys23.  


Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys23 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H2B (Lys23) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.   Anti-acetyl-Histone H2B (Lys23) mouse mAb
Anti-acetyl-Histone H2B (Lys23) mouse mAb 

A. Dot blotting analysis on indicated amount of acetylated H2B peptide at Lys23 (lane 1), acetylated H2B peptide at Lys20 (lane 2), butyrylated H2B peptide at Lys20 (lane 3), crotonylated H2B peptide at Lys20 (lane 4), 2ohibutyrylated H2B peptide at Lys20 (lane 5), 3ohbutyrylated H2B peptide at Lys20 (lane 6), butyrylated H2B peptide at Lys23 (lane 7), crotonylated H2B peptide at Lys23 (lane 8), 2ohibutyryl H2B peptide at Lys23 (lane 9), 3ohbutyrylated H2B peptide at Lys23 (lane 10), acetylated H2B peptide at Lys24 (lane11), 2ohibutyryl H2B peptide at Lys24 (lane 12), 3ohbutyrylated H2B peptide at Lys24 (lane 13), and unmodified H2B peptide at Lys23 (lane 14) using acetyl-histone H2B (Lys23) mouse mAb.  


 B. Western blotting analysis on 30 µg of crude proteins from HeLa whole cell lysates with (“+”) or without (“-“) treatment of Trichostatin A(500ng, 4 hours) using acetyl-histone H2B (Lys23) mouse mAb (1:2000).  


 C. Western blotting analysis on 30 μg of crude proteins from Rice histone lysate, N2a and Kidney whole cell lysates using acetyl-histone H2B (Lys23) mouse mAb (1:1000).


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC.



ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


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