修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H2B (Lys24) mouse mAb

Cat#:

PTM-169

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H2B (Lys24) peptide affinity chromatography. It specifically recognizes histone H2B with acetylation at Lys24.  

Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys24 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H2B (Lys24) peptide affinity chromatography.  

Specificity

H2B

Recommended Applications

ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation  


Recommended antibody dilution

WB: 1:2000  IP: 1:100

Important

 For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

Anti-acetyl-Histone H2B (Lys24) mouse mAb

A. Dot  blotting analysis on indicated amount of acetylated H2B peptide at Lys24 (lane 1),  2ohibutyryl H2B peptide at Lys24 (lane 2), 3ohbutyrylated  H2B peptide at Lys24 (lane 3), acetylated H2B peptide at Lys16  (lane 4), butyrylated H2B peptide at Lys16 (lane 5), acetylated H2B peptide at  Lys20 (lane 6), butyrylated H2B peptide at Lys20 (lane 7), crotonylated H2B  peptide at Lys20 (lane 8), 2ohibutyryl H2B peptide at Lys20 (lane 9),  3ohbutyrylated H2B peptide at Lys20 (lane 10), acetylated H2B peptide at Lys23  (lane 11), butyrylated H2B peptide at Lys23 (lane 12), crotonylated H2B peptide  at Lys23 (lane13), 2ohibutyryl H2B peptide at Lys23 (lane 14),  3ohbutyrylated H2B peptide at Lys23 (lane 15), succinylated H2B peptide at  Lys34 (lane16), crotonylated H2B peptide at Lys34 (lane17), 2ohibutyryl  H2B peptide at Lys34 (lane 18), 3ohbutyrylated H2B peptide at Lys34 (lane 19), and unmodified H2B peptide at Lys24 (lane 20) using acetyl-histone  H2B (Lys24) mouse mAb.  

 

B. Western blotting analysis on 30 μg of crude proteins  from HeLa whole cell lysates with (lane2) or without (lane1) treatment of sodium  butyrate (30 mM, 4 hours) using acetyl-histone H2B (Lys24) mouse mAb (1:2000). 

Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation  in multiple sites, termed as “histone code”. Lysine acetylation in core histones soccurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC. Avoid repeated eze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.  


**This product is intended for Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation  


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