修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H2B (Lys24) rabbit pAb

Cat#:

PTM-126

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated Histone H2B at Lys24 peptide affinity chromatography. It specifically recognizes Histone H2B with acetylation at Lys24.  

Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys24 of human Histone H2B. Antibodies are purified by protein A-conjugated agarose followed by acetylated Histone H2B (Lys24) peptide affinity chromatography.  

Specificity

H2B

Recommended Applications

ELISA, WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

  For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

  Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

  Anti-acetyl-Histone H2B (Lys24) rabbit pAb

A. Dot  blotting analysis on indicated amount of acetylated H2B peptide at Lys24 (lane  1), acetylated H2B peptide at Lys20 (lane 2), butyrylated H2B peptide at Lys20  (lane 3), crotonylated H2B peptide at Lys20 (lane 4), 2ohibutyrylated H2B peptide at Lys20  (lane 5), 3ohbutyrylated H2B peptide at Lys20 (lane 6), crotonylated H2B peptide at Lys23 (lane 7), 2ohibutyrylated H2B peptide at Lys23  (lane 8), 3ohbutyrylated H2B peptide at Lys23 (lane 9), 2ohibutyrylated H2B  peptide at Lys24 (lane 10), 3ohbutyrylated H2B peptide at Lys24  (lane 11), succinylated H2B peptide at Lys34 (lane 12), crotonylated H2B peptide at Lys34 (lane 13), 2ohibutyrylated H2B peptide at Lys34  (lane 14), 3ohbutyrylated H2B peptide at Lys34 (lane 15),  and unmodified H2B peptide at Lys24 (lane 16) using acetyl-Histone H2B (Lys24)  rabbit pAb.  


B. Western blotting analysis  on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using  acetyl-Histone H2B (Lys24) rabbit pAb (1:2000).  

Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity.The amino-terminal tails of core histones undergo lysine acetylationin multiple sites, termed as “histone code”.Lysine acetylation in core histone soccurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNAtranscription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Morethan 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs)have been identified to date, while the mechanistic details of substrateselection and site specificity of these enzymes remain unclear. The regulation of histonelysine acetylation status is impaired in the pathologies of cancerand other diseases and therefore, enzymes regulating histone lysine acetylationhave become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. 

   

**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA, WB; Not tested in other applications 

*WB=Western blotting


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!