修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H3 (Lys4) rabbit pAb

Cat#:

PTM-124

Ab Type:

P

Applications:

WB, Dot, ELISA, IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with acetylation at Lys4.  



Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H3 (Lys4) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA,WB, IHC; Not tested in other applications 

*WB=Western blotting

 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

 Anti-acetyl-Histone H3 (Lys4) rabbit pAb  

A. Dot  blotting analysis on indicated amount of acetylated H3 peptide at Lys4 (lane 1),  crotonylated H3 peptide at Lys4 (lane 2), acetylated peptide at Lys9 (lane 3),  butyrylated peptide at Lys9 (lane 4), crotonylated peptide at Lys9 (lane 5) and  unmodified H3 peptide at Lys4 (lane 6) using acetyl-histone H3 (Lys4) rabbit pAb.

  

B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of  sodium butyrate (30 mM, 4 hours) using acetyl-histone H3 (Lys4) rabbit pAb (1:2000).    


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity.The amino-terminal tails of core histones undergo lysine acetylationin multiple sites, termed as “histone code”.Lysine acetylation in core histone soccurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Morethan 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs)have been identified to date, while the mechanistic details of substrateselection and site specificity of these enzymes remain unclear. The regulation of histonelysine acetylation status is impaired in the pathologies of cancerand other diseases and therefore, enzymes regulating histone lysine acetylationhave become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA,WB, IHC; Not tested in other applications 

*WB=Western blotting


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