修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H4 (Lys8) mouse mAb

Cat#:

PTM-164

Ab Type:

M

Applications:

WB, Dot, ELISA, IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H4 (Lys8) peptide affinity chromatography. It specifically recognizes histone H4 with acetylation at Lys8.  


Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys8 of human histone H4. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H4 (Lys8) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, WB, IHC; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.  

 Anti-acetyl-Histone H4 (Lys8) mouse mAb  

A.Dot blotting analysis on indicated amount of acetylated H4 peptide at Lys8 (lane ), acetylated H4 peptide at Lys5 (lane2), butyrylated H4 peptide at Lys5 (lane3), crotonylated peptide at Lys5 (lane 4), butyrylated H4 peptide at Lys8 (lane5), crotonylated peptide at Lys8 (lane 6), acetylated H4 peptide at Lys12 (lane7), propionylated H4 peptide at Lys12 (lane8), butyrylated H4 peptide at Lys12 (lane 9), crotonylated peptide at Lys12 (lane 10) and unmodified H4 peptide at Lys8 (lane 11) using acetyl-histone H4 (Lys8) mouse pAb.  

 

B.Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using acetyl-histone H4 (Lys8) mouse pAb (1:2000).  


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli. It plays vital roles in the regulation of many cellular processes including chromatin dynamics, transcriptional activities, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H2B is primarily acetylated at Lys5, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The level of  histone lysine acetylation has been found to be impaired in cancers and other diseases, therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Wu Q,et al. SAHA treatment reveals the link between histone lysine acetylation and proteome in nonsmall cell lung cancer A549 Cells.J Proteome Res. 2013 Sep 6;12(9):4064-73. doi: 10.1021/pr4004079. Epub 2013 Aug 20.



ELISA, WB, IHC; Not tested in other applications 

*WB=Western blotting


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