修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H4 (Lys77) rabbit pAb

Cat#:

PTM-127

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated Histone H4 at Lys77 peptide affinity chromatography. It specifically recognizes Histone H4 with acetylation at Lys77.   

Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys77 of human Histone H4. Antibodies are purified by protein A-conjugated agarose followed by acetylated Histone H4 (Lys77) peptide affinity chromatography.  

Specificity

H4

Recommended Applications

ELISA, WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration.  Optimal dilutions/concentrations should be determined by the end user.  

 

 Anti-acetyl-Histone H4 (Lys77) rabbit pAb 

A. Dot  blotting analysis on indicated amount of acetylated H4 peptide at Lys77 (lane  1), propionylated H4 peptide at Lys77 (lane 2), succinylated H4 peptide  at Lys77 (lane 3), 2ohibutyrylated H4 peptide at Lys77 (lane 4),  acetylated H4 peptide at Lys79 (lane 5), propionylated H4 peptide at Lys79 (lane 6), butyrylated H4 peptide at Lys79 (lane  7), succinylated H4 peptide at Lys79 (lane 8), 2ohibutyrylated H4 peptide  at Lys79 (lane 9), and unmodified H4 peptide at Lys77 (lane 10) using acetyl-Histone  H4 (Lys77) rabbit pAb.  

 

B. Western blotting analysis  on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using  acetyl-Histone H4 (Lys77) rabbit pAb (1:2000). 

 

Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. 



**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  



ELISA, WB; Not tested in other applications 

*WB=Western blotting


推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!