修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H4 (Lys16) rabbit pAb

Cat#:

PTM-122

Ab Type:

P

Applications:

WB, Dot, ELISA, IHC

Species:

Human, Mouse, Rat, Rice

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H4 (Lys16) peptide affinity chromatography. It specifically recognizes histone H4 with acetylation at Lys16.  



 


Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys16 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H4 (Lys16) peptide affinity chromatography.  



 


Specificity

H4

Recommended Applications

ELISA,WB, IHC; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

 

 Anti-acetyl-Histone H4 (Lys16) rabbit pAb 


Anti-acetyl-Histone H4 (Lys16) rabbit pAb

A. Dot blotting analysis on indicated amount of acetylated H4 peptide at Lys16 (lane 1),  acetylated H4 peptide at Lys8 (lane 2), butyrylated H4 peptide at Lys8 (lane 3),  crotonylated peptide at Lys8 (lane 4), acetylated H4 peptide at Lys12 (lane 5),  propionylated H4 peptide at Lys12 (lane 6), butyrylated H4 peptide at Lys12  (lane 7), propionylated H4 peptide at Lys16 (lane8), butyrylated H4 peptide at Lys16  (lane 9), crotonylated peptide at Lys16 (lane 10), and unmodified H4 peptide at  Lys16 (lane 11) using acetyl-histone H4 (Lys16) rabbit pAb.


B. Western blotting analysis on 30 μg of crude proteins  from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of  sodium butyrate (30 mM, 4 hours) using acetyl-histone H4 (Lys16) rabbit pAb  (1:2000).  


C. Western blotting analysis on 30 μg of crude proteins from Rice histone lysate using acetyl-histone H4 (Lys16) rabbit pAb (1: 400). 


 


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity.The amino-terminal tails of core histones undergo lysine acetylationin multiple sites, termed as “histone code”.Lysine acetylation in core histone soccurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Morethan 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs)have been identified to date, while the mechanistic details of substrateselection and site specificity of these enzymes remain unclear. The regulation of histonelysine acetylation status is impaired in the pathologies of cancerand other diseases and therefore, enzymes regulating histone lysine acetylationhave become promising targets for anti-cancer drugs.  



 


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Wu Q,et al.SAHA treatment reveals the link between histone lysine acetylation and proteome in nonsmall cell lung cancer A549 Cells. J Proteome Res. 2013 Sep 6;12(9):4064-73. doi: 10.1021/pr4004079. Epub 2013 Aug 20.

ELISA,WB, IHC; Not tested in other applications 

*WB=Western blotting


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