修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H4 (Lys12) mouse mAb

Cat#:

PTM-165

Ab Type:

M

Applications:

WB, ELISA, ICC, IHC, ChIP

Species:

Human, Mouse, Rat

Price: (100 μl)

2500.00

Price: (20 μl)

 750.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by acetylated histone H4 (Lys12) peptide affinity chromatography. It specifically recognizes histone H4 with acetylation at Lys12.  


Source/Purification

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys12 of human histone H4. Antibodies are purified by protein G-conjugated agarose followed by acetylated histone H4 (Lys12) peptide affinity chromatography.  


Specificity

H4

Recommended Applications

ELISA, WB, ICC,  IHC; Not tested in other applications 

*WB=Western blotting    IHC=Immunohistochemistry    ICC=Immunocytochemistry

ChIP=Chromatin Immunoprecipitation


Recommended antibody dilution

WB: 1:2000; ICC: 1:200;  IHC: 1:200

Important

 For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.  

 

Anti-acetyl-Histone H4 (Lys12) mouse mAb

A. Acetyl-Histone H4 (Lys12) mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated acetyl-histone H4 (Lys12) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the acetyl-histone H4 (Lys12) peptide competed away binding of the antibody.  

B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using acetyl-histone H4 (Lys12) mouse mAb (1:2000).  

Anti-acetyl-Histone H4 (Lys12) mouse mAb

C. Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling H4K12ac with PTM-165 at 1/200 dilution. Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin.

Anti-acetyl-Histone H4 (Lys12) mouse mAb

D. Immunocytochemistry analysis

of  Hela (Human cervix adenocarcinoma cell line) untreated (Plane A) or treated with SBA (5mM 24h, Plane B) labeling  H4K12ac with PTM-165 at 1/100 dilution.

Cells were fixed with 4% paraformaldehyde and

permeabilized with 0.1% Triton X-100.

Anti-acetyl-Histone H4 (Lys12) mouse mAb

E. Chromatin Immunoprecipitation. Chromatin prepared from 1 x 105 HeLa cells treated with sodium butyrate was subjected to chromatin immunoprecipitation (ChIP) using either serial dilutions of acetyl-Histone H4 (Lys12) mouse mAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, GAPDH Promoter , RPL30 Exon 3 and MyoD1 Exon 1. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.




Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli. It plays vital roles in the regulation of many cellular processes including chromatin dynamics, transcriptional activities, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15, 16, and 20. Histone H2B is primarily acetylated at Lys5, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The level of  histone lysine acetylation has been found to be impaired in cancers and other diseases, therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Anti-di-methyl-Histone H3 (Lys9) rabbit pAb

D. Western blotting analysis on 30 µg of crude proteins from  Rice, Cerastium arvense  histone lysates, N2a and Spleen whole cell lysates  using di-methyl-histone H3 (Lys9) rabbit pAb (1:400).
Recommended Applications

ELISA, WB, ICC,  IHC; Not tested in other applications 

*WB=Western blotting    IHC=Immunohistochemistry    ICC=Immunocytochemistry

ChIP=Chromatin immunoprecipitation

Recommended antibody dilution

WB: 1:2000; ICC: 1:200;  IHC: 1:200


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