修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-acetyl-Histone H3 (Lys64) rabbit pAb

Cat#:

PTM-129

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated histone H3 (Lys64) peptide affinity chromatography. It specifically recognizes histone H3 with acetylation at Lys64.  

 

Source/Purification

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys64 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H3 (Lys64) peptide affinity chromatography.  

 

Specificity

H3

Recommended Applications

ELISA, WB; Not tested in other applications   

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

 

Anti-acetyl-Histone H3 (Lys64) rabbit pAb

 A. Dot  blotting analysis on indicated amount of acetylated H3 peptide at Lys64 (lane 1),  mono-methylated H3 peptide at Lys64 (lane 2), di-methylated H3 peptide at Lys64  (lane 3), tri-methylated H3 peptide at Lys64 (lane 4), acetylated H3 peptide at  Lys56 (lane 5), propionylated H3 peptide at Lys56 (lane 6), succinylated H3  peptide at Lys56 (lane 7), crotonylated H3 peptide at Lys56 (lane 8), 2ohibutyrylated  H3 peptide at Lys56 (lane 9), 3ohbutyrylated H3 peptide at Lys56 (lane 10), mono-methylated  H3 peptide at Lys56 (lane 11), di-methylated H3 peptide at Lys56 (lane 12), acetylated  H3 peptide at Lys79 (lane 13), butyrylated H3 peptide at Lys79 (lane 14), succinylated  H3 peptide at Lys79 (lane 15), crotonylated H3 peptide at Lys79 (lane 16), 2ohibutyrylated  H3 peptide at Lys79 (lane 17), 3ohbutyrylated H3 peptide at Lys79 (lane 18), mono-methylated  H3 peptide at Lys79(lane 19), di-methylated H3 peptide at Lys79 (lane 20),  tri-methylated H3 peptide at Lys79 (lane 21), and unmodified H3 peptide at Lys64  (lane 22) using acetyl-histone H3 (Lys64) rabbit pAb.  


 B. Western blotting analysis on 30 μg of crude proteins  from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of  sodium butyrate (30 mM, 4 hours) using acetyl-histone H3 (Lys64) rabbit pAb  (1:2000).  

Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.  


Storage & Stability

 Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.   


**This product is intended for Rresearch purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.  


ELISA, WB; Not tested in other applications   

*WB=Western blotting  


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