修饰抗体 Products
组蛋白精氨酸甲基化 (arg-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-di-methyl(asy)-Histone H3(Arg2) mouse mAb

Cat#:

PTM-668

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2500.00

Price: (20 μl)

750.00


Source/Purification

This product is produced by immunizing mice with a synthetic 2me(asy) peptide corresponding to residues surrounding Arg2 of human histone H3. Antibodies are purified by Protein G conjugated agarose followed by di-methylated (asy) H3 (Arg2) peptide affinity chromatography.


Recommended Applications

ELISA, Dot blot, Western blot. 

Recommended antibody dilution: WB: 1:2000

NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

 Anti-di-methyl(asy)-Histone H3(Arg2) mouse mAbAnti-di-methyl(asy)-Histone H3(Arg2) mouse mAb

 

Scientific Background
Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine rsidues on the amino-terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri- methylation on Lys, mono- di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation


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