修饰抗体 Products
组蛋白精氨酸甲基化 (arg-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-di-methyl(sy)-Histone H3 (Arg17) rabbit pAb

Cat#:

PTM-679

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2500.00

Price: (20 μl)

750.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by symmetric di-methylated histone H3 peptideat Arg17affinity chromatography. It specifically recognizes histone H3 with symmetric di-methylation at Arg17.  

Source/Purification

This product is produced by immunizing rabbitswith a synthetic di-methyl(sy) peptide corresponding to residues surrounding Arg17 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by di-methylated(sy) histone H3 (Arg17) peptide affinity chromatography.  

Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:25

Important

 For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

 Anti-di-methyl(sy)-Histone H3 (Arg17) rabbit pAb  

A. Dot blotting analysis on indicated amount of symmetric di-methylated H3 peptide  at Arg17 (lane 1), mono-methylated H3 peptide at Arg2 (lane 2), symmetric  di-methylated H3 peptide at Arg2 (lane 3), asymmetric di-methylated H3 peptide  at Arg2 (lane 4), mono-methylated H3 peptide at Arg8 (lane 5), symmetric di-methylated  H3 peptide at Arg8 (lane 6), asymmetric di-methylated H3 peptide at Arg8 (lane  7), mono-methylated H3 peptide at Arg17 (lane 8), asymmetric di-methylated H3  peptide at Arg17 (lane 9),symmetric di-methylated H3 peptide at Arg26 (lane  10), asymmetric di-methylated H3 peptide at Arg26 (lane 11), and unmodified H3 peptide at Arg17 (lane 12) using di-methyl(sy)-histone  H3 (Arg17)rabbit pAb.

 

B.Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12, MCF-7 whole cell lysates and 200 ng recombinant histone H3 proteins using symmetric di-methyl-histone H3 (Arg17)rabbit pAb (1:2000).

 

Scientific Description

Protein post-translational Modification (PTM) is an important reversible event controlling protein activity. The amino-terminal tails of core histones undergo multiple modifications in multiple sites, termed as “histone code” or “epigenetic code”. Arginine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species,arginine methylation occurs primarily on histones H3 (Arg2, 8, 17, 26) and H4 (Arg2) and has been implicated in both transcriptional activation and silencing.Arginine methylation in histones is carried out by the arginine N-methyltransferase (PRMT), which catalyzes the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine. Arginine methylation in histones is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone arginine methylation have become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt. 


**Research purposes only.Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation


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