修饰抗体 Products
组蛋白精氨酸甲基化 (arg-methylation) 修饰抗体
杭州景杰生物科技有限公司
Mono-methyl-Histone H3 (Arg42) rabbit pAb

Cat#:

PTM-610

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Arg42 of human histone H3. Antibodies are purified by protein A-conjugated agarosefollowed by mono-methylated histone H3 (Arg42) peptide affinity chromatography.


Source/Purification

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Arg42 of human histone H3. Antibodies are purified by protein A-conjugated agarosefollowed by mono-methylated histone H3 (Arg42) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA,Dot blot,Western blot. 


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

捕获14.JPG

捕获15.JPG

A.Dot blotting analysis on indicated amount of peptides using histone H3R42me rabbit pAb. The list of peptides is included in the table  on the right.


B.Western blotting analysis on 30 μg of crude proteins from MCF-7, C2C12, N2a (Figure B) and 30 μg of crude proteins from JK and BRL whole cell lysates (Figure C) using methyl-histone H3 (Arg42) rabbit pAb (1: 2000).

 

Scientific Description

Proteins post-translational Modification (PTM) is an important reversible event controlling protein activity. The amino-terminal tails of core histones undergo multiple modifications in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation or arginine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, arginine methylation occurs primarily on histones H3 (Arg2, 8, 17, 26) and H4 (Arg3) and has been implicated in both transcriptional activation and silencing. Arginine methylation in histones is carried out by the arginine N-methyltransferase (PRMT), which catalyzes the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine. Arginine methylation in histones is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone arginine methylation have become promising targets for anti-cancer drugs.


Storage & Stability

The antibody is delivered in powder form. Before use, please read carefully the Reconstitution and Storage Instructions. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 


1.Zhang K, Chen Y, Zhang Z,et al,J Proteome Res. 2009, 8(2):900-906

2. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.

3. Chen Y, Sprung R, Tang Y, et alMol Cell Proteomics. 2007, 6(5):812-819.


ELISA,Dot blot,Western blot. 



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