修饰抗体 Products
组蛋白赖氨酸单甲基化(mono-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-mono-methyl-Histone H3 (Lys4) rabbit pAb

Cat#:

PTM-611

Ab Type:

P

Applications:

ELISA,Dot, WB, ICC, IHC.

Species:

Human, Mouse, Rat, Arabidopsis thaliana, Cerastium

Price: (100 μl)

2500.00

Price: (20 μl)

750.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by mono-methylated histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with mono-methylation at Lys4.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by mono-methylated histone H3 (Lys4) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA,Dot, WB, ICC, IHC.

Not tested in other applications 

*WB=Western blotting    IHC=Immunohistochemistry    ICC=Immunocytochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:100    ICC: 1:200

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.    


Anti-mono-methyl-Histone H3 (Lys4) rabbit pAb
A. Dot  blotting analysis on indicated amount of mono-methylated H3 peptide at Lys4  (lane 1), di-methylated H3 peptide at Lys4 (lane 2), tri-methylated H3 peptide  at Lys4 (lane 3), mono-methylated H3 peptide at Lys9 (lane 4), di-methylated H3  peptide at Lys9 (lane 5), tri-methylated H3 peptide at Lys9 (lane 6), mono-  methyl ated H3 peptide at Lys14 (lane 7) , mono-methylated H3 peptide at Lys18  (lane8) and unmodified H3 peptide at Lys4 (lane 9) using mono-methyl-histone H3  (Lys4) rabbit pAb.

B.Western blotting analysis on 30 μg of crude proteins from HeLa, C2C12 and COS-7 whole cell lysates using mono-methyl-histone H3 (Lys4) rabbit pAb (1:2000).

 

Anti-mono-methyl-Histone H3 (Lys4) rabbit pAb

C. Western blotting analysis on 30 μg of crude proteins from Arabidopsis thaliana , Cerastium arvense  histone lysates, N2a and Spleen whole cell lysates  using mono-methyl-histone H3 (Lys4) rabbit pAb (1:2000).

Anti-mono-methyl-Histone H3 (Lys4) rabbit pAb

D. Immunohistochemistry analysisof paraffin-embedded mouse colon tissue, labeling H3K4me with PTM-611 at 1/100 dilution. Nuclear staining on epithelial cells of mouse colon was observed. Counter stained with hematoxylin.

Anti-mono-methyl-Histone H3 (Lys4) rabbit pAb

E. Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma cell line), labeling H3K4me with PTM-611 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Nuclei were stained with DAPI (blue).



Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



Recommended Applications

ELISA,Dot, WB, ICC, IHC.

Not tested in other applications 

*WB=Western blotting    IHC=Immunohistochemistry    ICC=Immunocytochemistry


Recommended antibody dilution

WB: 1:2000    IHC: 1:100    ICC: 1:200


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