修饰抗体 Products
组蛋白赖氨酸单甲基化(mono-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-mono-methyl-Histone H3 (Lys56) rabbit pAb

Cat#:

PTM-626

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

¥2500.00

Price: (20 μl)

750.00


Product Description

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Lys56 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by mono-methylated histone H3 (Lys56) peptide affinity chromatography.


Source/Purification

This product is produced by immunizing rabbits with a synthetic mono-methyl peptide corresponding to residues surrounding Lys56 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by mono-methylated histone H3 (Lys56) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA,WB; Not tested in other applications 

*WB=Western blotting


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Mono-methyl-Histone H3 (Lys56)rabbitpAb

A.Dot blotting analysis on indicated amount of mono-methylated H3 peptide at Lys56 (lane 1), acetylated H3 peptide at Lys56 (lane 2), di-methylated H3 peptide at Lys56 (lane 3), tri-methylatedH3 peptide at Lys56 (lane 4) and unmodified H3 peptideat Lys56 (lane 5)using mono-methyl-histone H3 (Lys56)rabbit pAb.


B.Western blotting analysis on 30 μg of crude proteins from HeLa,C2C12 and COS whole cell lysates using mono-methyl-histone H3 (Lys56)rabbit pAb(1:2000).

 

Scientific Description

The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for theformation of active and inactive regions of the genome and therefore playsvital roles in multiple cellular events. In most species, lysine methylationoccurs primarily on histones H3 (Lys4, 9, 27, 36, 79), and H4 (Lys20) and hasbeen implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs). The level of methyllysineis found impaired in the pathologies of cancers and other diseases, therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.


Storage & Stability

Store product at -20℃. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt. 


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA,WB; Not tested in other applications 

*WB=Western blotting



推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!