修饰抗体 Products
组蛋白赖氨酸单甲基化(mono-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-mono-methyl-Histone H2B (Lys15) mouse mAb

Cat#:

PTM-661

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by mono-methylated histone H2B (Lys15) peptide affinity chromatography. It specifically recognizes histone H2B with mono-methylation at Lys15.  


Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic mono-methyl peptide corresponding to residues surrounding Lys15 of human histone H2B. Antibodies are purified by protein G-conjugated agarose followed by mono-methylated histone H2B (Lys15) peptide affinity chromatography.  


Specificity

H2B

Recommended Applications

ELISA, WB, IP   Not tested in other applications  

*WB=Western blotting; IP=Immunoprecipitation


Recommended antibody dilution

WB: 1:2000 IP: 1:15~1:30

Important
For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications 

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Anti-mono-methyl-Histone H2B (Lys15) mouse mAb



Scientific Description

The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been associated with transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  


Storage & Stability

The antibody is delivered in powder form. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 

 

**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB, IP   Not tested in other applications  

*WB=Western blotting; IP=Immunoprecipitation


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