修饰抗体 Products
组蛋白赖氨酸二甲基化 (di-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-di-methyl-Histone H3 (Lys4) mouse mAb

Cat#:

PTM-641

Ab Type:

M

Applications:

WB, Dot, ELISA, IHC

Species:

Human, Mouse, Rat, Pharbitis

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The mouse-derived antibody is purified with protein G-conjugated agarose followed by di-methylated histone H3 (Lys4) peptide affinity chromatography. It specifically recognizes histone H3 with di-methylation at Lys4.  

Source/Purification

This product is produced by immunizing mice with a synthetic di-methyl peptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by di-methylated histone H3 (Lys4) peptide affinity chromatography.  

Specificity

H3

Recommended Applications

ELISA,WB, IHC; Not tested in other applications   

*WB=Western blotting  


Recommended antibody dilution

WB: 1:2000

Important

 For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.   

 Anti-di-methyl-Histone H3 (Lys4) mouse mAb  

Anti-di-methyl-Histone H3 (Lys4) mouse mAb

A. Dot  blotting analysis on indicated amount of di-methylated H3 peptide at Lys4 (lane  1), mono-methylated H3 peptide at Lys4 (lane 2), tri-methylated H3 peptide at Lys4  (lane 3), mono-methylated H3 peptide at Lys9 (lane 4), di-methylated H3 peptide  at Lys9 (lane5), tri-methylated H3 peptide (lane6) and unmodified H3 peptide at  Lys4 (lane 7) using di-methyl-histone H3 (Lys4) mouse mAb.


B. Western blotting analysis on 30 μg of crude proteins  from HeLa, 3T3 and MCF-7 whole cell lysates using di-methyl-histone H3 (Lys4) mouse  mAb (1:2000).  


C. Western blotting analysis on 30 μg of crude proteins from Rice , Pharbitis limbate histone lysates, N2a and Spleen whole cell lysates using histone H3K4me2 mouse mAb (1:2000).


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



ELISA,WB, IHC; Not tested in other applications   

*WB=Western blotting  


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