修饰抗体 Products
组蛋白赖氨酸二甲基化 (di-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-di-methyl-Histone H3 (Lys36) mouse mAb

Cat#:

PTM-662

Ab Type:

M

Applications:

WB, Dot, ELISA,IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description
Source/Purification
This product is produced by immunizing Balb/c mice with a synthetic histone H3 peptide which is di-methylated at Lys36. Antibodies are purified by protein G-conjugated agarose.


Specificity

H3

Recommended Applications

 ELISA,WB; IHC.

Not tested in other applications

 *WB=Western blotting


 *WB=Western blotting
Recommended antibody dilution

WB: 1:2000

Important

For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.  

捕获2.JPG
A:Dot blotting analysis on indicated amount of peptides using histone H3K36me2 mouse mAb. The list of peptides is included in right table.  

捕获1.JPG

B:Western blotting analysis on 30 μg of crude proteins from MCF-7, BRL, C2C12, COS-7, N2a whole cell lysates using anti-H3K36me2 mouse mAb (1:2000).   
Scientific Description
The ε-amino lysine methylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been associated with transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  
Storage & Stability

The antibody is delivered in powder form. Before use, please read carefully the Reconstitution andStorage Instructions. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 


 ELISA,WB,IHC; Not tested in other applications

 *WB=Western blotting


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