修饰抗体 Products
组蛋白赖氨酸三甲基化 (tri-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-tri-methyl-Histone H3 (Lys23) rabbit pAb

Cat#:

PTM-638

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

¥ 2500.00

Price: (20 μl)

750.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by tri-methylated histone H3 (Lys23) peptide affinity chromatography. It specifically recognizes histone H3 with tri-methylation at Lys23.  


Source/Purification

This product is produced by immunizing rabbits with a synthetic tri-methyl peptide corresponding to residues surrounding Lys23 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by tri-methylated histone H3 (Lys23) peptide affinity chromatography.  


Specificity

H3

Recommended Applications

ELISA, Dot blot,WB; Not tested in other applications

*WB=Western blotting; 

 
 

Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  


Anti-tri-methyl-Histone H3 (Lys23) rabbit pAb

No.
Peptide No.Peptide
Lane 1H3K23me3Lane 2H3K18me3
Lane 3H3K23meLane 4H3K23me2

Figure A: Dot blotting analysis on indicated 

amount of peptides using H3K23me3 rabbit pAb. 

The list of peptides is included in the table on the

right.

Lane 5H3K27me3Lane 6H3K23un

Anti-tri-methyl-Histone H3 (Lys23) rabbit pAb

Anti-tri-methyl-Histone H3 (Lys23) rabbit pAb

Figure B: Western blotting analysis on 30 μg of crude 

proteins from human HeLa, mouse N2a, rat BRL whole cell 

lysates using anti-H3K23me3 rabbit pAb (1: 2000). 

Figure C: Western blotting analysis on 30 μg of crude 

proteins from mouse Liver and mouse Kidney whole tissue

lysates  using anti-H3K23me3 rabbit pAb (1: 2000)


Anti-tri-methyl-Histone H3 (Lys23) rabbit pAb


 Figure D: Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates using H3K23me3 rabbit pAb (1:2000) pre-absorbed with 1.5 μM H3K23me3 peptides (lane 1), unmodified peptides (lane 2) or without peptide (lane 3). The antibodies were completely competed with the H3K23me3 peptides  but not with unmodified peptides.


Scientific Description

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methylases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.  


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA, WB; Not tested in other applications

*WB=Western blotting; 

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