修饰抗体 Products
组蛋白赖氨酸三甲基化 (tri-methylation) 修饰抗体
杭州景杰生物科技有限公司
Tri-methyl(Lys4)/ serotonyl(Gln5)-Histone H3 rabbit pAb (H3K4me3/Q5ser pAb)

Cat#:

PTM-1420

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2500.00

Price: (20 μl)

¥ 750.00


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Lys4 and Gin5 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by peptide affinity chromatography.



Recommended Applications

ELISA, Dot blot, Western blot.

Recommended antibody dilution: WB: 1:2000


Important

For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at

an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.



Specificity: 


 Figure A:Dot blot

Tri-methyl(Lys4)/ serotonyl(Gln5)-Histone H3 rabbit pAb (H3K4me3/Q5ser pAb)


Dot blotting analysis on indicated amount of peptides using H3K4me3/Q5ser rabbit pAb. The list

of peptides is included in the table on the right.



 Figure B:Western blot

 Tri-methyl(Lys4)/ serotonyl(Gln5)-Histone H3 rabbit pAb (H3K4me3/Q5ser pAb)

Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse N2a and rat BRL whole cell lysates and mouse kidney whole tissue lysates using H3K4me3/Q5ser rabbit pAb (1:2000).


Figure C:Western blot

Tri-methyl(Lys4)/ serotonyl(Gln5)-Histone H3 rabbit pAb (H3K4me3/Q5ser pAb)


Western blotting analysis on 30 μg of crude proteins from mouse N2a whole cell lysates using H3K4me3/Q5ser rabbit pAb (1:1000) pre-absorbed with 1.5 μM H3K4me3/Q5ser peptides (lane 1), unmodified peptides (lane 2) or without peptide (lane 3). The antibodies were completely competed with the H3K4me3/Q5ser peptides but not with other peptides.


Scientific Description

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. Serotonin, also known as 5-hydroxytryptamine

(5-HT), is an excitatory neurotransmitter that regulates brain cell activity by activating specific membrane receptors in the brain. The amino group of serotonins can covalently bind to the glutamine residue at the fifth position of histone H3 by TGM2 to form histone 5-hydroxytryptamine (H3Q5ser). H3Q5ser modifies sites adjacent to H3K4me3 form PTM crosstalk. The presence of H3K4me3Q5ser enhanced the binding of

transcription factors, such as TFIID to chromatin, thereby activating the expression of downstream genes in developing rodent brains and human neurons.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



Farrelly et al, Nature, 2019

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