修饰抗体 Products
组蛋白赖氨酸三甲基化 (tri-methylation) 修饰抗体
杭州景杰生物科技有限公司
Tri-methyl-Histone H3(Lys14) mouse mAb

Cat#:

PTM-646

Ab Type:

M

Applications:

WB, Dot, ELISA,IHC

Species:

Human, Mouse, Rat

Price: (100 μl)

2500

Price: (20 μl)

750.00


Source/Purification

This product is produced by immunizing Balb/c mice with a synthetic tri-methyl peptide corresponding to residues surrounding Lys14 of human histone H3. Antibodies are purified by protein G-conjugated agarose followed by tri-methylated histone H3 (Lys14) peptide affinity chromatography.


Specificity

H3

Recommended Applications

ELISA, Dot blot, Western blot,IHC.

Recommended antibody dilution: WB: 1:2000


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

图片3.png

A. Dot blotting analysis on indicated amount of peptides using histone H3K14me3 mouse mAb. The list of peptides is included in the table on the right.


B. Western blotting analysis on 30 µg of crude proteins from N2a, HeLa and MCF-7 whole cell lysates and mouse spleen tissue lysate using the tri-methyl-histone H3 (Lys14) mouse mAb (1: 2000).


Scientific Description

Proteins post-translational Modification (PTM) is an important reversible event controlling protein activity. The amino-terminal tails of core histones undergo multiple modifications in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation or arginine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, arginine methylation occurs primarily on histones H3 (Arg2, 8, 17, 26) and H4 (Arg3) and has been implicated in both transcriptional activation and silencing. Arginine methylation in histones is carried out by the arginine N-methyltransferase (PRMT), which catalyzes the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine. Arginine methylation in histones is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone arginine methylation have become promising targets for anti-cancer drugs.


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.



ELISA, Dot blot, Western blot,IHC.

Recommended antibody dilution: WB: 1:2000


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